Journal: PLoS ONE

Article Title: Plaque Size Is Decreased but M1 Macrophage Polarization and Rupture Related Metalloproteinase Expression Are Maintained after Deleting T-Bet in ApoE Null Mice

doi: 10.1371/journal.pone.0148873

Figure Lengend Snippet: Primary (A) and secondary (B) antibodies and Griffonia simplicifolia Lectin I (GSL).

Article Snippet: , GSL I , VectorLabs, USA B1215 , Lectin , 1:100.

Techniques: Concentration Assay

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Maternal and fetal alkaline ceramidase 2 is required for placental vascular integrity in mice

doi: 10.1096/fj.202001104R

Figure Lengend Snippet: A-D, Tissue sections were prepared from E12.5 fetoplacental units from Acer2−/− or Acer2+/+ dams (n=5) bred to Acer2−/− and Acer2+/+ males, respectively, as described in Materials and Methods. The tissue sections close to the placental midline were stained with a PAS solution (A and B) or were subjected to ISH analysis using a RNAscope probe specific for the gene Prl2c2 (C and D) or Tpbpa (G and H), or were labeled with the biotinylated lectin IB4 (I and J). The areas stained with the RNAscope probes were shown in red and those stained with the IB4 lection in brown. The P-TGC numbers (E) and the areas of the P-TGC layer (F) and Jz ( K) and capillary areas (L) were quantified using the software Fiji. The images in B, D, H, and J were zoomed from the regions framed by the yellow rectangles in A, C, G, and I, respectively. Data are presented as means ± SD and statistically analyzed by one-way ANOVA. *, p <0.05 vs. indicated group.

Article Snippet: Sections were stained with 2 μg/ml of biotinylated Griffonia simplicifolia lectin I isolectin B4 (IB4) (Cat.# B-1205, Vector Laboratories, Burlingame, CA) in PBS, followed by HRP-conjugated streptavidin (1:4000) (Thermo-Fisher).

Techniques: Staining, Labeling, Software

Journal:

Article Title: Aponecrotic, antiangiogenic and antiproliferative effects of a novel dextran derivative on breast cancer growth in vitro and in vivo

doi: 10.1038/sj.bjp.0704645

Figure Lengend Snippet: Inhibition of MCF-7ras tumour angiogenesis by NaPaC, CMDB and NaPa. Microvessel staining of tumours untreated (A) and treated with 150 mg kg−1 CMDB (B), 40 mg kg−1 NaPa (C) or 15 mg kg−1 NaPaC (D), twice a week, for 7 weeks, was performed using GSL-1 lectin. Necrotic areas in panel d are indicated with the asterisks. Magnification ×250 was used for panels A, B and C, and magnification ×100 was applied for panel D. The representative microvessels in panels A – C are marked with the arrows.

Article Snippet: Endothelial cells were specifically labelled with GSL-1 isolectin B4 (Vector Laboratories, Burlingame, CA, U.S.A.).

Techniques: Inhibition, Staining